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Derivation of a human blastocyst after heterologous nuclear transfer to donated oocytes M Stojkovic1,3, P Stojkovic1, C Leary2, VJ Hall1, L Armstrong1, M Herbert2, M Nesbitt2, M Lako1, A Murdoch2 In this paper we describe the derivation of a blastocyst following heterologous nuclear transfer (NT) into a human oocyte. We also demonstrate that a major obstacle to continuing research in human NT is the availability of suitable human oocytes. In this study, 36 oocytes were donated by 11 women undergoing 4 different treatments and we evaluated their developmental potential after NT procedure. The time from oocyte collection to NT seems to be crucial and we only had success with oocytes which were enucleated within 1 hour. After enucleation of oocytes, fusion with undifferentiated human embryonic stem cells and in-vitro culture we observed early cleavage and blastocyst development of fused complexes. The DNA fingerprinting comparison of the donor cells and derived blastocyst revealed successful heterologous NT since both oocytes and donor cells were recovered from different patients. We have therefore demonstrated that NT can be achieved in the human using heterologous donor nuclei and surplus and donated oocytes. However, if the promise of this new science is to achieve its potential in the foreseeable future, we will need to identify new sources of oocytes that can be used immediately after retrieval.
Keywords: egg donation, embryo, nuclear transfer, oocyte, stem cells
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